Ashish Jain

Gyanveer University, India

Title: Insulin loaded Amino acid conjugated PLGA nanoparticles for management of type 1 diabetes

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Biography

Jain studied Pharmaceutical sciences at the renowned department of pharmaceutical sciences, Dr. Hari Singh Gour University, Sagar, India and completed post graduation as M.Pharm. in 2006, received his PhD degree in 2014. He worked three years as research associate. Dr. Ashish has got to his credit four book chapters and more than 25 papers published in international journals and many are communicated in international journals of repute. He has presented research paper in two international conferences held in “Austria & Ireland” with BRG grant. He obtained the position of Head & Professor at the Gyanveer University, Sagar.

Abstract

Oral delivery is the preferred route of administration because it offers several advantages over other routes. However, it is not an effective route for the delivery of peptides and proteins because of so many constraints. Insulin is degraded in the gut of GIT because of presence of proteolytic enzyme. The small intestine has been shown to be able to transport the L-forms of amino acids against a concentration gradient and that they compete for the mechanism concerned. So L-Valine was used as a ligand for carrier mediated transport of insulin loaded PLGA nanoparticles. Ex-vivo studies on intestine revealed that conjugated nanoparticles showed greater insulin uptake as compared to nonconjugated nanoparticles. In-vivo studies were performed on streptozotocin induced diabetic rabbits.  Oral suspension of insulin loaded PLGA nanoparticles reduced blood glucose level from 265.4±8.5 to 246.6±2.4 mg/dl within 4 hrs which further decreased to 198.7±7.1 mg/dl value after 8 hrs, The ligand conjugated formulation on oral administration produced hypoglycaemic effect within 4 hrs of administration, the hypoglycaemic effect prolonged till 12 hours of oral administration. Hence, it is concluded that the L-valine conjugated NPs bearing insulin are the promising carrier for the transportation of insulin across the intestine on oral administration.